permaculture

[Steve Hart <stevenlawrencehart@gmail.com>]

Hi Declan...yes I did my masters in bed after breaking my back..a great way
to focus

By the way..there is plenty of those mongrels operating within the
Permaculture world too.

Steve Hart

On 5 July 2016 at 09:53, scrooge McDuck <declan@planetmail.net> wrote:

> Well the reading I have done & stuff watched since my stroke has turned me
> off GMOs big time. One of the few advantages of the stroke was that I
> arrived in the 21st century. I discovered Permaculture. At night I watched
> documentaries and torrents I had downloaded during the day rather than
> suffer trash tv. So I became aware of the damage mankind is doing, and the
> inherent evil of large super profitable businesses who focus on today's
> profits rather than tomorrow's planet.
>
> --
> Declan Moriarty
>
> Sent from my Android tablet with mail.com Mail. Please excuse my brevity.
> On 04/07/2016, 21:20 Steve Hart <stevenlawrencehart@gmail.com> wrote:
>
>> Yes Declan..it is another diversion from nature. What did we learn in
>> Permaculture ? ? One that rings in my ears " work with nature not against
>> her" because as we know...Mother nature always fights back. I've just
>> started re-reading "Hidden Nature" by Bartholomew a biography on
>> Schauberger...a must for all in the Permaculture network...Steve Hart
>>
>> On 5 July 2016 at 00:52, Lawrence London <lfljvenaura@gmail.com> wrote:
>>
>> > On Mon, Jul 4, 2016 at 5:45 AM, scrooge McDuck <declan@planetmail.net>
>> > wrote:
>> >
>> > > What often horrifies me is where they get these 'crisper genes' from -
>> > > often from sources we would do more than raise an eyebrow at. I have
>> few
>> > > facts, but I'm not comfortable with what I do know.
>> > >
>> > > Regards
>> > >
>> > > Declan Moriarty.
>> > >
>> > > *Sent:* Sunday, July 03, 2016 at 10:13 PM
>> > > *From:* "Lawrence London" <lfljvenaura@gmail.com>
>> > > *To:* permaculture <permaculture@lists.ibiblio.org>
>> > > *Subject:* [permaculture] Crisper gene technology - are there
>> > > (John d'Hondt: Will this new powerful gene modification technology
>> have
>> > > implications for permaculture, changing the genetic makeup pf
>> generations
>> > > of organisms, including those in the wild?)
>> > >
>> > > Crisper gene technology - Google Search
>> > >
>> >
>> https://www.google.com/search?q=Crisper+gene+technology&ie=utf-8&oe=utf-8
>> > >
>> >
>> > From Wikipedia:
>> > https://en.wikipedia.org/wiki/CRISPR
>> > CRISPR
>> > From Wikipedia, the free encyclopedia
>> > <https://en.wikipedia.org/wiki/File:Crispr.png>
>> > Diagram of the CRISPR prokaryotic antiviral defense mechanism.[1]
>> > <https://en.wikipedia.org/wiki/CRISPR#cite_note-pmid20056882-1>
>> >
>> > *Clustered regularly interspaced short palindromic repeats* (*CRISPR*,
>> > pronounced *crisper*[2]
>> > <https://en.wikipedia.org/wiki/CRISPR#cite_note-sawyer2013-2>) are
>> > segments
>> > of prokaryotic <https://en.wikipedia.org/wiki/Prokaryotic> DNA
>> > <https://en.wikipedia.org/wiki/DNA> containing short repetitions of
>> base
>> > sequences. Each repetition is followed by short segments of "spacer DNA
>> > <https://en.wikipedia.org/wiki/Spacer_DNA>" from previous exposures to
>> a
>> > bacteriophage virus or plasmid <https://en.wikipedia.org/wiki/Plasmid
>> >.[3]
>> > <https://en.wikipedia.org/wiki/CRISPR#cite_note-pmid20125085-3>
>> >
>> > The *CRISPR/Cas system* is a prokaryotic immune system
>> > <https://en.wikipedia.org/wiki/Immune_system> that confers resistance
>> to
>> > foreign genetic elements such as those present within plasmids
>> > <https://en.wikipedia.org/wiki/Plasmids> and phages
>> > <https://en.wikipedia.org/wiki/Phage>,[4]
>> > <https://en.wikipedia.org/wiki/CRISPR#cite_note-4>[5]
>> > <https://en.wikipedia.org/wiki/CRISPR#cite_note-pmid17379808-5>[6]
>> > <https://en.wikipedia.org/wiki/CRISPR#cite_note-pmid19095942-6> and
>> > provides a form of acquired immunity
>> > <https://en.wikipedia.org/wiki/Acquired_immunity>. CRISPR spacers
>> > recognize
>> > and cut these exogenous genetic elements in a manner analogous to RNA
>> > interference <https://en.wikipedia.org/wiki/RNA_interference> in
>> > eukaryotic
>> > <https://en.wikipedia.org/wiki/Eukaryote> organisms.[3]
>> > <https://en.wikipedia.org/wiki/CRISPR#cite_note-pmid20125085-3> CRISPRs
>> > are
>> > found in approximately 40% of sequenced bacterial
>> > <https://en.wikipedia.org/wiki/Bacterial> genomes and 90% of sequenced
>> > archaea <https://en.wikipedia.org/wiki/Archaea>.[7]
>> > <https://en.wikipedia.org/wiki/CRISPR#cite_note-pmid17521438-7>[note 1]
>> > <https://en.wikipedia.org/wiki/CRISPR#cite_note-8>
>> >
>> > By delivering the Cas9 <https://en.wikipedia.org/wiki/Cas9> nuclease
>> and
>> > appropriate guide RNAs into a cell, the cell's genome
>> > <https://en.wikipedia.org/wiki/Genome> can be cut at a desired
>> location,
>> > allowing existing genes to be removed and/or new ones added.[8]
>> > <https://en.wikipedia.org/wiki/CRISPR#cite_note-nature99-9>[9]
>> > <https://en.wikipedia.org/wiki/CRISPR#cite_note-vb99-10>[10]
>> > <https://en.wikipedia.org/wiki/CRISPR#cite_note-pmid26121415-11>
>> CRISPRs
>> > have been used in concert with specific endonuclease
>> > <https://en.wikipedia.org/wiki/Endonuclease> enzymes
>> > <https://en.wikipedia.org/wiki/Enzymes> for genome editing
>> > <https://en.wikipedia.org/wiki/Genome_editing> and gene regulation in
>> > species throughout the tree of life
>> > <https://en.wikipedia.org/wiki/Tree_of_life_%28biology%29>.[11]
>> > <https://en.wikipedia.org/wiki/CRISPR#cite_note-12>
>> >
>> > Cas9 was the first nuclease discovered, followed by Cpf1, which was
>> > discovered in the CRISPR/Cpf1 <
>> https://en.wikipedia.org/wiki/CRISPR/Cpf1>
>> > system of Francisella novicida
>> > <https://en.wikipedia.org/wiki/Francisella_novicida>.[12]
>> > <https://en.wikipedia.org/wiki/CRISPR#cite_note-pmid26422227-13>[13]
>> > <https://en.wikipedia.org/wiki/CRISPR#cite_note-pmid27096362-14> Other
>> > such
>> > systems are thought to exist.[14]
>> > <https://en.wikipedia.org/wiki/CRISPR#cite_note-:3-15>
>> >
>> > CRISPR/C2c2 from the bacterium Leptotrichia shahii
>> > <https://en.wikipedia.org/wiki/Fusobacteria> is RNA-guided CRISPR
>> system
>> > that targets RNA rather than DNA, and can either cleave single-stranded
>> RNA
>> > targets or knock them down <
>> https://en.wikipedia.org/wiki/Gene_knockdown>.
>> > [15] <https://en.wikipedia.org/wiki/CRISPR#cite_note-pmid27256883-16>
>> >
>> > The CRISPR interference <
>> https://en.wikipedia.org/wiki/CRISPR_interference
>> > >
>> > technique has many potential applications, including altering the
>> germline
>> > <https://en.wikipedia.org/wiki/Germline> of humans, animals, and food
>> > crops. The use of CRISPR for genome editing[16]
>> > <https://en.wikipedia.org/wiki/CRISPR#cite_note-17>[17]
>> > <https://en.wikipedia.org/wiki/CRISPR#cite_note-18> was the AAAS
>> > <
>> >
>> https://en.wikipedia.org/wiki/American_Association_for_the_Advancement_of_Science
>> > >'s
>> > choice for breakthrough of the year in 2015.[18]
>> > <https://en.wikipedia.org/wiki/CRISPR#cite_note-19> Bioethical
>> > <https://en.wikipedia.org/wiki/Bioethical> concerns have been expressed
>> > about the prospect of using this nascent biotechnology for editing the
>> > human germline.[19] <https://en.wikipedia.org/wiki/CRISPR#cite_note-20>
>> > <>
>> > Bitter fight over CRISPR patent heats up : Nature News & Comment
>> > <
>> >
>> http://www.nature.com/news/bitter-fight-over-crispr-patent-heats-up-1.17961
>> > >
>> > www.nature.com/news/bitter-fight-over-*crispr*-patent-heats-up-1.17961
>> > Nature Jan 12, 2016 -
>> > Use of the *technology* in research has exploded, thanks to
>> *CRISPR*–Cas9's
>> > relative simplicity and versatility compared to other gene-editing ...
>> > <>
>> > http://www.radiolab.org/story/antibodies-part-1-crispr/
>> > Hidden inside some of the world’s smallest organisms is one of the most
>> > powerful tools scientists have ever stumbled across. It's a defense
>> system
>> > that has existed in bacteria for millions of years and it may some day
>> let
>> > us change the course of human evolution.
>> > <>
>> >
>> > I listened to an interview on public radio last week with an (the?)
>> > inventor of the CRISPR technology currently in intense development.
>> > 1) He insisted and will campaign for development and use of CRISPR to be
>> > completely in the public view, interactive with the public and not
>> behind
>> > closed doors. He said that this is the way forward for maximum progress
>> > with and best use of CRISPR.
>> > 2) He described it this way. With conventional gene splcing the GMO
>> > organism can eventually shed the modifications to its genetic makeup.
>> With
>> > CRISPR it can not.because it inserts a gene segment plus a tool (spacer)
>> > that keeps it in place and protects it from evolutionary changes. In
>> other
>> > words it makes permanent changes to an organism's genome such that, as
>> any
>> > organism modified with CRISPR reproduces, those changes are retained in
>> any
>> > offspring and for generations forward. [this is my take on what he said
>> > several days ago - I tried to find the interview and name of the
>> researcher
>> > but without luck],
>> >
>> > So, Declan, what "questionable" materials are sourced for use in CRISPR.
>> > BTW they are talking about modifications to the human genome with this
>> > promising technology. .
>> > --
>> > Lawrence F. London
>> > lfljvenaura@gmail.com
>> > https://sites.google.com/site/avantgeared/
>> > https://plus.google.com/u/0/b/116100117925365792988/+Avantgeared/posts
>> > https://ello.co/permaculture
>> > https://lists.ibiblio.org/sympa/arc/permaculture
>> > permaculture mailing list
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>> >
>>
>>
>>
>> --
>> Kia ora Ehoa
>>
>> [image: Picture]
>> *Steve Hart*
>> *Ecology Architect*
>> *Designer, Teacher, Builder of Ecologically Sustainable Resilient
>> Environments, through Urban Design, Architecture, Landscape Architecture
>> and Systems Ecology.​*
>> Skype: tipenemanawa
>> Phone: +64 220 75 62 11
>>
>> Permaculture Global Design:
>> www.permacultureglobaldesign.org
>>
>> The Permaculture International College
>> www.permaculturecollege.eu
>> https://permaculturecollege.academia.edu/SteveHart
>>
>> GAPPS The Global Alliance for Permaculture Partnerships and Solutions.
>> www.permaculturepartnerships.com
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-- 
Kia ora Ehoa

[image: Picture]
*Steve Hart*
*Ecology Architect*
*Designer, Teacher, Builder of Ecologically Sustainable Resilient
Environments, through Urban Design, Architecture, Landscape Architecture
and Systems Ecology.​*
Skype: tipenemanawa
Phone: +64 220 75 62 11

Permaculture Global Design:
www.permacultureglobaldesign.org

The Permaculture International College
www.permaculturecollege.eu
https://permaculturecollege.academia.edu/SteveHart

GAPPS The Global Alliance for Permaculture Partnerships and Solutions.
www.permaculturepartnerships.com