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[permaculture] Fwd: Intracellular colonization of roots of Arabidopsis and crop plants by Gluconacetobacter diazotrophicus - Springer
- From: Lawrence London <lfljvenaura@gmail.com>
- To: permaculture <permaculture@lists.ibiblio.org>
- Subject: [permaculture] Fwd: Intracellular colonization of roots of Arabidopsis and crop plants by Gluconacetobacter diazotrophicus - Springer
- Date: Thu, 1 Aug 2013 01:07:36 -0400
Intracellular colonization of roots of *Arabidopsis* and crop plants
by *Gluconacetobacter
diazotrophicus*
- Edward C.
Cocking<http://link.springer.com/search?facet-author=%22Edward+C.+Cocking%22>,
- Philip J.
Stone<http://link.springer.com/search?facet-author=%22Philip+J.+Stone%22>,
- Michael R.
Davey<http://link.springer.com/search?facet-author=%22Michael+R.+Davey%22>
http://link.springer.com/article/10.1079%2FIVP2005716
Summary
We have investigated the interaction of *Gluconacetobacter diazotrophicus*,
a non-nodulating endophytic nitrogen-fixing bacterium isolated from the
intercellular spaces of sugarcane, with *Arabidopsis thaliana* and the crop
plants maize (*Zea mays*), rice (*Oryza sativa*), wheat (*Triticum aestivum*),
oilseed rape (*Brassica napus*), tomato (*Lycopersicon esculentum*), and
white clover (*Trifolium repens*). Using seedlings grown aseptically in
sucrose-containing culture media, we have shown that inoculation with very
low numbers of *G. diazotrophicus* results in extensive intracellular
colonization of root meristems and progressive systemic intracellular root
colonization. Light microscopic examination of thin sections of
resin-embedded root tips of *Arabidopsis* and these crop plants inoculated
with β-glucuronidase (GUS)-labeled and with NifH promoter-GUS-labeled *G.
diazotrophicus* showed blue-stained *G. diazotrophicus* within the
cytoplasm of root cells, indicating that intracellular conditions were
suitable for nitrogenase gene expression. Electron microscopy confirmed
that these bluestained intracellular *G. diazotrophicus* were within
membrane-bounded vesicles. We discuss whether these novel inoculations with
*G. diazotrophicus* are likely to enable non-nodular endosymbiotic nitrogen
fixation and whether these inoculations can also provide a plant system to
investigate the endosymbiotic theory of the origin of eukaryotic organelles.
- [permaculture] Fwd: Intracellular colonization of roots of Arabidopsis and crop plants by Gluconacetobacter diazotrophicus - Springer, Lawrence London, 08/01/2013
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