[permaculture] Fwd: [SANET-MG] Grass Plants Bind, Retain, Uptake, and Transport Infectious Prions

Lawrence London lfljvenaura at gmail.com
Mon May 18 15:45:32 EDT 2015

---------- Forwarded message ----------
From: Terry S. Singeltary Sr. <flounder9 at verizon.net>
Date: Mon, May 18, 2015 at 2:39 PM
Subject: Re: [SANET-MG] Grass Plants Bind, Retain, Uptake, and Transport
Infectious Prions
To: SANET-MG at lists.ifas.ufl.edu

<<<With all that one would expect numbers in domestic and wild ruminants to
go up exponentially. Is there anything known about that Terry?

give it time, it's the silent epidemic $$$

in my opinion John, they have not a clue. the OIE testing criteria is not
set up to find BSE or any TSE prion in my opinion, it's really set up to
NOT find BSE, do to the simple fact of the numbers they are testing, or the
lack there of. they simply are not testing enough. once the atypical BSE
cases started showing up, usda shut the testing back down to numbers they
can't find BSE with, unless it's by an accident. and really, who cares
about a disease that is 100% fatal once clinical, a disease that incubates
for so long, you can never hardly trace back anything from it, whether it
be animal, people or product. case in point, 10,000,000 pounds of banned
blood laced ruminant protein that was fed out into commerce in 2007, some
10 years post mad cow feed ban. how's that working out for us$

if you look at cwd in the cervid, it's spreading at a pretty good pace in
North America, and does not look like it can be stopped yet. as far as the
bovine and bse, impossible to know since the OIE and the USDA made the TSE
Prion a legal global trading commodity by the BSE MRR policy, when they did
away with the BSE GBR policy. the testing there is at such a small number,
they have to just about find bse aka mad cow disease by accident, and
that's if the testing is done properly. how many times has the usda et al
botched BSE testing, let me count the ways. you could argue the same thing
with scrapie. there trying to pen the atypical BSE strains on a spontaneous
event, when there is no such science to prove it, all the while ignoring
what's taking place in France with atypical BSE. please understand, one of
the greatest threats that has not come to pass yet, by documentation, if
ever documented, would be a worst case nightmare. _if_ or _when_, a strain
of the tse prion transmits to the bovine (or human), that can transmit
vertical and horizontal in such a way as scrapie and cwd does in cervid and
caprine, then we are in a world of hurt. to date, as far as I know, the
only bovine tse prion tested for such a thing has been the typical mad cow
strain i.e. the c-BSE, i.e. the (typical c-BSE UK strain, as they term it),
I think they believe a small amount is maternal, and now we have another
study here about the placenta in scrapie.

Friday, April 24, 2015

The placenta shed from goats with classical scrapie is infectious to goat
kids and lambs



Mother to offspring transmission of chronic wasting disease in Reeve's
Muntjac deer

Amy Nalls,1 Erin McNulty,1 Jenny Powers,2 Davis Seelig,1 Clare Hoover,1
Nicholas Haley,1 Jeanette Hayes-Klug,1 Kelly Anderson,1 Paula Stewart,3
Wilfred Goldmann,3 Edward A. Hoover1 and Candace K. Mathiason1 1Colorado
State University; Fort Collins, CO USA; 2National Park Service; Fort
Collins, CO USA; 3The Roslin Institute and Royal School of Veterinary
Studies; Edinburgh, UK

To investigate the role mother to offspring transmission plays in chronic
wasting disease (CWD), we have developed a cervid model employing the
Reeve's muntjac deer (Muntiacus reevesi). Eight muntjac doe were orally
inoculated with CWD and tested PrPCWD lymphoid positive by 4 mo post
infection. Fourteen fawns were born to these eight CWD-infected doe-3 were
born viable, 6 were born non-viable and 5 were harvested as fetuses from
early or end-stage CWD-infected doe. All three viable fawns have
demonstrated CWD IHC lymphoid biopsy positivity between 43 d post birth and
11 mo post birth. Two of these three CWD positive viable offspring have
developed clinical signs consistent with TSE disease (28-33 mo post birth).
Moreover, CWD prions have been detected by sPMCA in 11 of 16 tissues
harvested from 6 full-term non-viable fawns and in 7 of 11 fetal tissues
harvested in utero from the second and third trimester fetuses. Additional
tissues and pregnancy related fluids from doe and offspring are being
analyzed for CWD prions. In summary, using the muntjac deer model we have
demonstrated CWD clinical disease in offspring born to CWD-infected doe,
and in utero transmission of CWD from mother to offspring. These studies
provide basis to further investigate the mechanisms of maternal transfer of




but they have not a clue yet about the atypical BSE strains, and this is
very worrisome, especially with the atypical L-type BASE BSE. ...

see ;

older study ;


56. Members considered that there is no evidence that crops grown on the
land which received composted excreta from BSE-challenged animals pose a
TSE risk to humans or animals. One member suggested that, as some of these
animals are orally challenged with high doses of BSE-infected materials,
and the distribution of infectivity in the digestive system is not
completely understood, it might be premature to conclude that there is no
infective agent in the manure.

Furthermore, an unpublished study had indicated low level absorption of PrP
from soil by tomato plants although it should be noted that this study had
not been repeated. Details of this work would be sent to the SEAC
Secretary. Dr Matthews explained that most of the manure from animals
challenged with high doses of BSE had already been composted and used for
coppicing. Members agreed that the risks from disposal of residual manure
from experimental animals would be much less than historic risks of on farm
contamination from naturally infected animals at the height of the BSE
epidemic. ...SNIP...END





Protocol for further laboratory investigations into the distribution of
infectivity of Atypical BSE1


Information on the pathogenesis and tissue distribution of Atypical Bovine
Spongiform Encephalopathy (BSE) in cattle through the study of field cases
and experimental transmission studies is lacking. The latter are limited to
transmission of Atypical BSE through intracerebral (i.c.) inoculation of
cattle. All data currently available relate to the presence or absence of
PrPSc, but do not quantify relative amounts of PrPSc or levels of
infectivity. A laboratory protocol for further studies is recommended, to
allow the assessment of the relative infectious titre, PrPSc accumulation
and prion seeding activity in the tissues of cattle that developed H-BSE or
L-BSE (using posterior brainstem as a reference). Tissues to be covered by
those studies are categorised in three priorities, based on their inclusion
in the list of specific risk material in cattle, on the presence of
infectivity, or PrPSc presence, demonstrated in Atypical BSEs or other
Transmissible Spongiform Encephalopathies (TSEs) in ruminants, and on the
importance in terms of input into the food chain in the EU. The protocol
provides details in terms of the minimum number of animals to be tested,
processing and preparation of tissues, and methods to be used to identify
abnormal PrP and quantify infectivity, also depending on the expected level
of infectivity and amount of tissue available for analysis. It is
recommended that, through the implementation of the protocol, information
should also be obtained on the performance of currently validated rapid
tests for TSE active surveillance in cattle/bioassay for detecting H-BSE
and L-BSE agents.

© European Food Safety Authority, 2014


Atypical BSE, cattle, H-BSE, L-BSE, laboratory protocol, prion


To date, 27 cases of L-BSE and 24 cases of H-BSE have been report­ed
worldwide (16), thus meaning that the prevalence of atypical BSE is
considerably lower than that of C-BSE. However, recent studies showed that
L-BSE is easily transmissible to transgenic mice expressing human (17,18)
or bovine (19,20) prion protein, as well as to non-human primates (21),
with shorter incubation periods than for the transmission of C-BSE to these
animals. The virulent nature of L-BSE has stimulated new concern for human
public health since the transmis­sion of C-BSE to humans resulted in
variant Creutz­feldt-Jakob disease (vCJD) (4-7), a new emergent prion


All the cases of BSE identified during the major outbreak in the UK were of
the same strain type [19]. However, an atypical form of BSE, Bovine
Amyloidotic Spongiform Encephalopathy (BASE), was discovered in Italy in
2004 in two old (11 and 15 year old) asymptomatic cows post mortem [19].
Other atypical forms of BSE were subsequently reported in France, Germany
and Japan [19-22]. The frequency of atypical BSE may be similar to the
occurrence of sporadic CJD, which is about 1 per million individuals [23].
BASE can be biochemically differentiated from BSE by the different mobility
of PrP fragments on gel electrophoresis. BASE can also be distinguished
from BSE histo-pathologically based on differences in the distribution of
vacuoles in the brain. ***BASE shares molecular and histopathological
features with the MV2 sub-type of human sporadic Creutzfeldt-Jakob Disease


spontaneous atypical BSE ???

if that's the case, then France is having one hell of an epidemic of
atypical BSE, probably why they stopped testing for BSE, problem solved $$$

As of December 2011, around 60 atypical BSE cases have currently been
reported in 13 countries, *** with over one third in France.


spontaneous events of mad cow disease $$$

so 20 cases of atypical BSE in France, compared to the remaining 40 cases
in the remaining 12 Countries, divided by the remaining 12 Countries, about
3+ cases per country, besides Frances 20 cases. you cannot explain this
away with any spontaneous BSe. ...TSS

Sunday, October 5, 2014

France stops BSE testing for Mad Cow Disease


Identification of a second bovine amyloidotic spongiform encephalopathy:
Molecular similarities with sporadic Creutzfeldt–Jakob disease

snip...please remember, the last home grown case of mad cow disease in the
USA, that was documented (this is key, that was _documented_), was in
California, and it was a case of the atypical L-type BASE BSE. please see ;

Saturday, May 09, 2015

*** Expression of genes involved in the T cell signalling pathway in
circulating immune cells of cattle 24 months following oral challenge with
Bovine Amyloidotic Spongiform Encephalopathy (BASE) ***


Thursday, April 30, 2015

*** Immediate and ongoing detection of prions in the blood of hamsters and
deer following oral, nasal, or blood inoculations


Friday, January 30, 2015

*** Scrapie: a particularly persistent pathogen ***


Tuesday, December 16, 2014

Evidence for zoonotic potential of ovine scrapie prions


Although Bovine Spongiform Encephalopathy (BSE) is the cause of variant
Creutzfeldt Jakob disease (vCJD) in humans, the zoonotic potential of
scrapie prions remains unknown. Mice genetically engineered to overexpress
the human ​prion protein (tgHu) have emerged as highly relevant models for
gauging the capacity of prions to transmit to humans. These models can
propagate human prions without any apparent transmission barrier and have
been used used to confirm the zoonotic ability of BSE. Here we show that a
panel of sheep scrapie prions transmit to several tgHu mice models with an
efficiency comparable to that of cattle BSE. The serial transmission of
different scrapie isolates in these mice led to the propagation of prions
that are phenotypically identical to those causing sporadic CJD (sCJD) in
humans. These results demonstrate that scrapie prions have a zoonotic
potential and raise new questions about the possible link between animal
and human prions.


why do we not want to do TSE transmission studies on chimpanzees $

5. A positive result from a chimpanzee challenged severly would likely
create alarm in some circles even if the result could not be interpreted
for man. I have a view that all these agents could be transmitted provided
a large enough dose by appropriate routes was given and the animals kept
long enough. Until the mechanisms of the species barrier are more clearly
understood it might be best to retain that hypothesis.




Thursday, March 26, 2015

National Scrapie Eradication Program Monthly Report - February 2015


what about manipulating tse prion testing to suit your needs $$$

Thursday, November 18, 2010


Dustin Douglass was indicted and charged with making a fraudulent
application to the VA, in an effort to obtain benefits from injuries
Douglas represented he suffered while deployed in Iraq. Based on his
application, the VA provided benefits totaling $22,148.53. Douglass claimed
he suffered various injuries and illnesses as a result of his service in
combat. The investigation revealed Douglass had, in fact, been deployed to
Iraq, but had served as a computer specialist, had never been in combat,
and did not suffer the service-related injuries and illnesses he claimed to
have suffered. Douglass was placed on supervised release for 3 years, and
required to pay $22,148.53 in restitution. Galen Niehues, an inspector for
the Nebraska Department of Agriculture, (NDA), was convicted of mail fraud
for submitting falsified reports to his employer concerning inspections he
was supposed to perform at Nebraska cattle operations. Niehues was tasked
with performing inspections of Nebraska ranches, cattle and feed for the
presence of neurological diseases in cattle including Bovine Spongiform
Encephalopathy (BSE), also known as “Mad Cow Disease”. Niehues was to
identify cattle producers, perform on-site inspections of the farm sites
and cattle operations, ask producers specific questions about feed, and
take samples of the feed. Niehues was to then submit feed samples for
laboratory analysis, and complete reports of his inspections and submit
them to the NDA and to the Federal Food and Drug Administration (FDA). An
investigation by the FDA and NDA revealed Niehues had fabricated
approximately 100 BSE inspections and inspection reports. When confronted,
Niehues admitted his reports were fraudulent, and that had fabricated the
reports and feed samples he submitted to the NDA. Niehues received a
sentence of 5 years probation, a 3-year term of supervised release, and was
required to pay $42,812.10 in restitution.



and what about these famous tse prion tests, just what did happen here, ask
the redacted part that was uncovered in the second part...an epic ten year
battle to find the truth...the damn sheep never did have a tse prion...and
they knew it...tss

Friday, February 20, 2015

APHIS Freedom of Information Act (FOIA) Appeal Mouse Bio-Assays
2007-00030-A Sheep Imported From Belgium and the Presence of TSE Prion
Disease Kevin Shea to Singeltary 2015


Saturday, January 31, 2015

RAPID ADVICE 17-2014 : Evaluation of the risk for public health of casings
in countries with a “negligible risk status for BSE” and on the risk of
modification of the list of specified risk materials (SRM) with regard to


Sunday, April 12, 2015

*** Research Project: Transmission, Differentiation, and Pathobiology of
Transmissible Spongiform Encephalopathies 2014 Annual Report ***

April 5, 2015*** Guidance for Industry Ensuring Safety of Animal Feed
Maintained and FedOn-Farm Draft Guidance FDA-2014-D-1180 ***
the true numbers of victims from the mad cow crisis, is not
beingdocumented, due to the industry fed nvCJD only theory, a theory that
has nowbeen proven wrong. sporadic CJD has now been linked to atypical
BSE,atypical Scrapie, and typical scrapie, with cwd waiting for
science...Sunday, November 23, 2014*** Confirmed Variant Creutzfeldt-Jakob
Disease (variant CJD) Case in Texasin June 2014 confirmed as USA case NOT
European ***
as predicted...tssSaturday, May 09, 2015*** Psychiatric Symptoms in
Patients With Sporadic Creutzfeldt-Jakob Diseasein Germany
Jan 2015 at 20:14 GMT*** Ruminant feed ban for cervids in the United
States? ***31 Jan 2015 at 20:14 GMThttp://
www.plosone.org/annotation/listThread.action?root=8535119 May 2010 at 21:21
GMT*** Singeltary reply ; Molecular, Biochemical and Genetic
Characteristics ofBSE in Canada Singeltary reply ;
Replication of Ab Oligomers Suggests PotentialTransmissibility in Alzheimer
DiseaseReceived July 24, 2014; Accepted September 16, 2014; Published
November 3,2014Singeltary comment ;
January 24, 2015*** Bovine Spongiform Encephalopathy: Atypical Pros and
December 1, 2014Germany Bovine Spongiform Encephalopathy BSE
CJD TSE Prion disease A ReviewDecember 1, 2014
January 29, 2015Identification of H-type BSE in Portugalhttp://
January 29, 2015OIE REPORT Bovine spongiform encephalopathy Prion (atypical
BSE type H),Norway Information received on 29/01/2015
July 24, 2014Protocol for further laboratory investigations into the
distribution ofinfectivity of Atypical BSE SCIENTIFIC REPORT OF EFSAhttp://
3625-32000-086-05 Studyof Atypical Bsehttp://
Prominent Transmissible SpongiformEncephalopathy PRION disease Scientist in
the world, that was on this board,recently resigned do to the fact that the
PRION 2015 conference oralabstracts, and poster abstracts, are now NOT
available to the public, onlypay-per-view. this was a terrible loss not
only to this board, but to thescientific community tse prion world.
...tssWednesday May 2714:45 Jean-Phillipe Deslys Atomic Energy Commission,
France,Transmission of prions to primates after extended silent incubation
POPULATIONS.16:45Quingzhong Kong Case Western Reserve UniversityZoonotic
Potential of CWD Prionshttps://
prion2015.files.wordpress.com/2015/03/detailedschedule2.pdfSunday, May 3,
2015PRION2015 FORT COLLINShttp://
2015 all pay per view nowPRION20?? this is terrible. I’m sad. pay per view
now on the TSE Prionconferences $
how can I keep up?Prion 2015 Poster AbstractsPurchase optionsPrice *( )
Issue PurchaseUSD 189.00( ) Article PurchaseUSD 48.00
2015 Oral AbstractsPurchase optionsPrice *( ) Issue PurchaseUSD 189.00( )
Article PurchaseUSD 48.00
May 3, 2015*** PRION2015 FORT COLLINS ***
goes the neighborhood.tss-----Original Message-----From: John D'hondtSent:
Saturday, May 16, 2015 2:29 PMTo: SANET-MG at LISTS.IFAS.UFL.EDUSubject: Re:
[SANET-MG] Grass Plants Bind, Retain, Uptake, and TransportInfectious
PrionsWith all that one would expect numbers in domestic and wild ruminants
togo up exponentially. Is there anything known about that Terry?JohnOn
16/05/15 02:40, Terry S. Singeltary Sr. wrote:> price of Prion Poker goes
up $$$>>> Friday, May 15, 2015>> Grass Plants Bind, Retain, Uptake, and
Transport Infectious Prions>> Report>>
I’m thinking liquid or dried blood meal for land spreading, and the
TSEprion disease. ...slap me silly...tss>>>>
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Department of Agriculture- National Institute of Food and Agriculture
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expressed within do not necessarily reflect the view of the SARE program or
the U.S. Department of Agriculture. USDA is an equal opportunity provider
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Lawrence F. London
lfljvenaura at gmail.com
Ello: @ecoponderosa <https://ello.co/ecoponderosa>

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