North American Fruit Explorers mailing list at ibiblio

[Kristine Naess <kristine.naess@lesbuissons.qc.ca>]

Hello Jerry,

 

It would be neat to try the method you mention in tissue culture using meristems instead of whole buds.  A grad student at the university of MD tried to obtain graft induced chimeras in the early 90's by using in vitro techniques.  She was trying to produce a "hand in glove" type apple chimera which would have the outer cell layer from a scab resistant apple variety and the inner cell layers from a high quality apple susceptible to scab.  She was also trying to produce a thornfree blackberry hybrid in the same way.  There are thornfree Rubus which are natural hand in glove type chimeras and need to be propagated by stem cuttings rather than root cuttings in order to maintain thornlessness.

 

Kristine 

----- Original Message -----

From: Jwlehman@aol.com

To: nafex@lists.ibiblio.org

Sent: Saturday, February 19, 2005 8:05 AM

Subject: [NAFEX] Chimera

Hello,

I'm a little hesitant to way in on this subject as there are writers more educated on this subject than I.

One way to create a chimera is called Mentoring. However I've read two different definitions of mentoring. This definition would entail making two preciously identical cut scions ready for bark grafting. One of species "a" and the other of species "b" of which the chimera is to be made.  Then each prepared scion would be split lengthwise down the middle through the bud with a very sharp thin instrument such as a razor blade. From these, two bark grafts could be made, one a + b the other b + a. These are then brought together so the bud halves are perfectly aligned and inserted as bark grafts. If one is extremely lucky, and the bud halves don't die and they heal together then grow you could get a chimera.

I hope I've written this such that it is understandable. I've never tried it but have been tempted to using American and kaki persimmon.

Jerry

---

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