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[Homestead] Kitchen table (or closet) tissue culture operation
- From: tvoivozhd <tvoivozd AT infionline.net>
- To: homestead AT lists.ibiblio.org
- Subject: [Homestead] Kitchen table (or closet) tissue culture operation
- Date: Sun, 16 Jan 2005 17:28:24 -0500
Basic need is to reduce airborne contaminants, and reduce them further with a HEPA filter. A good way is to make and work inside a plastic tent.
http://www.omnisterra.com/botany/cp/slides/tc/tc.htm (kitchen table tissue culture)
Go for the plants that seem too expensive---blueberries would be a good choice, or outside the garden and orchard category, expensive flowers.
http://www.kitchenculturekit.com/kitchenMess.jpg (graphic of one version of a kitchen tissue culture lab.)
http://carnegieinstitution.org/first_light_case/horn/PTC/preptcmed.html (Forget the autoclave, do your own sterilizing in a pressure cooker)
http://home.neo.rr.com/kuska/holmes_hap.htm (homemade clean-air setup---total cost about $40)
http://www.tissuecon.com/aboutus2.htm (Indian website on bag culture, other cheap innovations)
http://www.kitchenculturekit.com/SUPPLY.HTM (you need stuff like balance scales, PH meter--for latter a cheap garden meter might work)
http://www.une.edu.au/%7Eagronomy/AgSSrHortTCinfo.html (good Australian instructions)
http://www.plantsci.cam.ac.uk/Haseloff/techniques/TechnicalPDFs/LowCostTCprac.pdf (more low-cost advice)
http://www.omnisterra.com/botany/cp/slides/tc/hood.htm (John Laroche glove box, test tube or baby food jar rotator---you need this, a fine reference)
- [Homestead] Kitchen table (or closet) tissue culture operation, tvoivozhd, 01/16/2005
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