From lfljvenaura at gmail.com Fri Feb 22 18:16:31 2019 From: lfljvenaura at gmail.com (Lawrence London) Date: Fri, 22 Feb 2019 18:16:31 -0500 Subject: [pchealth] Beans may be the very best thing you can eat for your microbiome. Here is why. : nutrition Message-ID: [also raw potato is excellent for your microbiome, is prebiotic and feeds beneficial intestinal microflora] Beans may be the very best thing you can eat for your microbiome. Here is why. : nutrition https://www.reddit.com/r/nutrition/comments/athyam/beans_may_be_the_very_best_thing_you_can_eat_for/?st=jsgo80ul&sh=9b52c3ee Introduction: "First off beans are the cornerstone for every blue zone diet in the world https://www.bluezones.com/2016/06/10-things-about-beans/ So there's that. But for the microbiome they are incredible. First understand how important Resistant starch is for the microbiome. https://www.dermveda.com/articles/why-you-should-eat-resistant-starch-for-a-healthy-microbiome Resistant starch is metabolized by microbes in the gut to produce molecules called short chain fatty acids, such as acetate, butyrate and propionate. These short chain fatty acids have significant health benefits, including cancer fighting benefits and anti-inflammatory effects.[4,5] Diets high in resistant starch lead to higher levels of short chain fatty acids and may offer protection against colon inflammation and disease.[6] The dietary fiber that is fermented by our gut microorganisms are referred to as ?prebiotics,? leading to the production of beneficial short chain fatty acids and other molecules, including serotonin and deoxycholic acid.[7,8] Eating resistant starch may even improve problems with insulin regulation, including insulin resistance and diabetes.[9] https://www.gutmicrobiotaforhealth.com/en/new-review-explores-latest-knowledge-resistant-starch-nutrition-modulator-gut-microbiota/ In addition, RS (resistant starch) has specific beneficial effects on the gut environment including increased populations of Ruminococcus bromii -a dominant member of the phylum Firmicutes that plays a primary role in releasing energy from dietary starches that escape digestion by host enzymes through its exceptional activity against particulate resistant starches. Besides this, ingestion of food products rich in RS has been shown to increase luminal short-chain fatty acid levels, modulate microbial metabolism and improve markers of glucose homeostasis and insulin sensitivity Regarding cell signalling pathways that can explain physiological effects of RS, using animal models it has been well documented that *resistant starch has strong anti-inflammatory properties* And beans are far and away the best source of resistant starch in our diets. They have many more times the RS as any other commonly eaten food. See here for more. https://www.hsph.harvard.edu/nutritionsource/2015/11/16/ask-the-expert-legumes-and-resistant-starch/ Eating beans may even alter the microbiome in such a way as to kill cancer cells! https://www.healio.com/gastroenterology/oncology/news/online/%7B1d9afc8b-cef1-4b45-94bc-61dbfc08144b%7D/rice-bran-navy-beans-boost-gut-microbiome-slow-crc-cell-growth To evaluate the chemopreventive effects of rice bran and navy bean intake, Ryan and colleagues performed a phase 2 pilot study (the BENEFIT trial), in which they randomly assigned 29 CRC survivors to consume 30 grams of rice bran per day, 35 grams of navy bean powder per day, or neither for 4 weeks. They found that increased consumption of rice bran and navy beans led to higher dietary levels of fiber, iron, zinc, thiamin, niacin, vitamin B6, folate, and alpha-tocopherol. Further, stool microbiome analyses revealed the rice bran group had increased gut microbial richness and diversity, and the navy bean group showed increased gut microbial richness. Notably, analysis of human stool metabolite extracts before and after the dietary intervention showed increased intake of rice bran or navy beans reduced growth of colorectal cancer cells. There's more but thats all I will post for now." -------------- next part -------------- An HTML attachment was scrubbed... URL: From lfljvenaura at gmail.com Fri Feb 22 18:21:15 2019 From: lfljvenaura at gmail.com (Lawrence London) Date: Fri, 22 Feb 2019 18:21:15 -0500 Subject: [pchealth] Beans may be the very best thing you can eat for your microbiome. Here is why. : nutrition In-Reply-To: References: Message-ID: A comment: I'm not sure why the discussion of the microbiome always focuses on fiber as amino acids are fermented by bacteria and produce benificial SCFA https://www.sciencedirect.com/science/article/pii/S1075996497901219 Abstract The abilities of slurries of human faecal bacteria to ferment 20 different amino acids were investigated in batch culture incubations. Ammonia, short chain fatty acids, and in some cases, amines, were the principal products of dissimilatory metabolism. The types of SCFA produced were dependent on the chemical compositions of the test substrates. Thus, acetate and butyrate were formed from the acidic amino acid glutamate, while acetate and propionate predominated in aspartate fermentations. Breakdown of the basic amino acids lysine and arginine was rapid, and yielded butyrate and acetate, and ornithine and citrulline, respectively. The major products of histidine deamination were also acetate and butyrate. However, fermentation of sulphur-containing amino acids was slow and incomplete. Acetate, propionate and butyrate were formed from cysteine, whereas the main products of methionine metabolism were propionate and butyrate. The simple aliphatic amino acids alanine and glycine were fermented to acetate, propionate and butyrate, and acetate and methylamine, respectively. Branched-chain amino acids were slowly fermented by colonic bacteria, with the main acidic products being branched-chain fatty acids one carbon atom shorter than the parent amino acid. Low concentrations of amines were also detected in these fermentations. Aliphatic-hydroxy amino acids were rapidly deaminated by large intestinal microorganisms. Serine was primarily fermented to acetate and butyrate, while threonine was mainly metabolised to propionate. Proline was poorly utilized by intestinal bacteria, but hydroxyproline was efficiently fermented to acetate and propionate. The aromatic amino acids tyrosine, phenylalanine and tryptophan were broken down to a range of phenolic and indolic compounds. Interestingly the by product of microbial breakdown of most of the AA was SCFA, which are suspected to be very beneficial to gut health among other things. https://www.frontiersin.org/articles/10.3389/fmicb.2016.00185/full On Fri, Feb 22, 2019 at 6:16 PM Lawrence London wrote: > [also raw potato is excellent for your microbiome, is prebiotic and feeds > beneficial intestinal microflora] > > Beans may be the very best thing you can eat for your microbiome. Here is > why. : nutrition > > https://www.reddit.com/r/nutrition/comments/athyam/beans_may_be_the_very_best_thing_you_can_eat_for/?st=jsgo80ul&sh=9b52c3ee > > Introduction: > > "First off beans are the cornerstone for every blue zone diet in the world > > https://www.bluezones.com/2016/06/10-things-about-beans/ > > So there's that. But for the microbiome they are incredible. First > understand how important Resistant starch is for the microbiome. > > > https://www.dermveda.com/articles/why-you-should-eat-resistant-starch-for-a-healthy-microbiome > > Resistant starch is metabolized by microbes in the gut to produce > molecules called short chain fatty acids, such as acetate, butyrate and > propionate. These short chain fatty acids have significant health benefits, > including cancer fighting benefits and anti-inflammatory effects.[4,5] > Diets high in resistant starch lead to higher levels of short chain fatty > acids and may offer protection against colon inflammation and disease.[6] > The dietary fiber that is fermented by our gut microorganisms are referred > to as ?prebiotics,? leading to the production of beneficial short chain > fatty acids and other molecules, including serotonin and deoxycholic > acid.[7,8] > > Eating resistant starch may even improve problems with insulin regulation, > including insulin resistance and diabetes.[9] > > > https://www.gutmicrobiotaforhealth.com/en/new-review-explores-latest-knowledge-resistant-starch-nutrition-modulator-gut-microbiota/ > > In addition, RS (resistant starch) has specific beneficial effects on the > gut environment including increased populations of Ruminococcus bromii -a > dominant member of the phylum Firmicutes that plays a primary role in > releasing energy from dietary starches that escape digestion by host > enzymes through its exceptional activity against particulate resistant > starches. Besides this, ingestion of food products rich in RS has been > shown to increase luminal short-chain fatty acid levels, modulate microbial > metabolism and improve markers of glucose homeostasis and insulin > sensitivity > > Regarding cell signalling pathways that can explain physiological effects > of RS, using animal models it has been well documented that *resistant > starch has strong anti-inflammatory properties* > > And beans are far and away the best source of resistant starch in our > diets. They have many more times the RS as any other commonly eaten food. > See here for more. > > > https://www.hsph.harvard.edu/nutritionsource/2015/11/16/ask-the-expert-legumes-and-resistant-starch/ > > Eating beans may even alter the microbiome in such a way as to kill cancer > cells! > > > https://www.healio.com/gastroenterology/oncology/news/online/%7B1d9afc8b-cef1-4b45-94bc-61dbfc08144b%7D/rice-bran-navy-beans-boost-gut-microbiome-slow-crc-cell-growth > > To evaluate the chemopreventive effects of rice bran and navy bean intake, > Ryan and colleagues performed a phase 2 pilot study (the BENEFIT trial), in > which they randomly assigned 29 CRC survivors to consume 30 grams of rice > bran per day, 35 grams of navy bean powder per day, or neither for 4 weeks. > > They found that increased consumption of rice bran and navy beans led to > higher dietary levels of fiber, iron, zinc, thiamin, niacin, vitamin B6, > folate, and alpha-tocopherol. Further, stool microbiome analyses revealed > the rice bran group had increased gut microbial richness and diversity, and > the navy bean group showed increased gut microbial richness. > > Notably, analysis of human stool metabolite extracts before and after the > dietary intervention showed increased intake of rice bran or navy beans > reduced growth of colorectal cancer cells. > > There's more but thats all I will post for now." > > -- Lawrence F. London, Jr. lfljvenaura at gmail.com https://sites.google.com/site/avantgeared -------------- next part -------------- An HTML attachment was scrubbed... URL: From lfljvenaura at gmail.com Tue Feb 26 08:24:18 2019 From: lfljvenaura at gmail.com (Lawrence London) Date: Tue, 26 Feb 2019 08:24:18 -0500 Subject: [pchealth] Aged Garlic Extract Modifies Human Immunity. - PubMed - NCBI Message-ID: Aged Garlic Extract Modifies Human Immunity. - PubMed - NCBI https://www.ncbi.nlm.nih.gov/pubmed/26764332 - NCBI[image: NCBI Logo] - Skip to main content - Skip to navigation - Resources - How To - About NCBI Accesskeys PubMed US National Library of Medicine National Institutes of Health Search database Search term [image: Clear input] - Advanced - Help Result Filters - Format: Abstract Send to J Nutr. 2016 Feb;146(2):433S-436S. doi: 10.3945/jn.115.210427. Epub 2016 Jan 13. Aged Garlic Extract Modifies Human Immunity. Percival SS 1. Author information Abstract Garlic contains numerous compounds that have the potential to influence immunity. Immune cells, especially innate immune cells, are responsible for the inflammation necessary to kill pathogens. Two innate lymphocytes, ??-T and natural killer (NK) cells, appear to be susceptible to diet modification. The purpose of this review was to summarize the influence of aged garlic extract (AGE) on the immune system. The author's laboratory is interested in AGE's effects on cell proliferation and activation and inflammation and to learn whether those changes might affect the occurrence and severity of colds and flu. Healthy human participants (n = 120), between 21 and 50 y of age, were recruited for a randomized, double-blind, placebo-controlled parallel-intervention study to consume 2.56 g AGE/d or placebo supplements for 90 d during the cold and flu season. Peripheral blood mononuclear cells were isolated before and after consumption, and ??-T and NK cell function was assessed by flow cytometry. The effect on cold and flu symptoms was determined by using daily diary records of self-reported illnesses. After 45 d of AGE consumption, ??-T and NK cells proliferated better and were more activated than cells from the placebo group. After 90 d, although the number of illnesses was not significantly different, the AGE group showed reduced cold and flu severity, with a reduction in the number of symptoms, the number of days participants functioned suboptimally, and the number of work/school days missed. These results suggest that AGE supplementation may enhance immune cell function and may be partly responsible for the reduced severity of colds and flu reported. The results also suggest that the immune system functions well with AGE supplementation, perhaps with less accompanying inflammation. This trial was registered at clinicaltrials.gov as NCT01390116 . ? 2016 American Society for Nutrition. KEYWORDS: NK cell; aged garlic extract; colds; flu; human immunity; ??-T cell PMID:26764332DOI:10.3945/jn.115.210427 [Indexed for MEDLINE] - - - Publication types, MeSH terms, Substances, Secondary source ID LinkOut - more resources - Supplemental Content Full text links [image: Icon for Silverchair Information Systems] Save items Similar articles - Supplementation with aged garlic extract improves both NK and ??-T cell function and reduces the severity of cold and flu symptoms: a randomized, double-blind, placebo-controlled nutrition intervention. [Clin Nutr. 2012] - Specific formulation of Camellia sinensis prevents cold and flu symptoms and enhances gamma,delta T cell function: a randomized, double-blind, placebo-controlled study. [J Am Coll Nutr. 2007] - Consumption of cranberry polyphenols enhances human ??-T cell proliferation and reduces the number of symptoms associated with colds and influenza: a randomized, placebo-controlled intervention study. [Nutr J. 2013] - Review Garlic Lowers Blood Pressure in Hypertensive Individuals, Regulates Serum Cholesterol, and Stimulates Immunity: An Updated Meta-analysis and Review. [J Nutr. 2016] - Review Efficacy and safety of Echinaforce? in respiratory tract infections. [Wien Med Wochenschr. 2013] See reviews... See all... Cited by 1 PubMed Central article - Review Interventions to restore appropriate immune function in the elderly. [Immun Ageing. 2018] Related information - Articles frequently viewed together - MedGen - Cited in PMC Recent Activity ClearTurn Off - Aged Garlic Extract Modifies Human Immunity. PubMed See more... 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URL: From lfljvenaura at gmail.com Tue Feb 26 08:25:25 2019 From: lfljvenaura at gmail.com (Lawrence London) Date: Tue, 26 Feb 2019 08:25:25 -0500 Subject: [pchealth] Healthy eating and exercise. Message-ID: Grow your own foods in a natural biointensive minimum till raised bed garden, fertilized and remineralized with quarry rock dusts, rock phosphate, azomite, greensand and seaweed, manures, compost tea, fermented plant extracts and more to produce nutrient dense vegetables ad fruits. Have a sprout farm: sprouted seeds in jars and buckwheat and sunflower sprouts grown in trays with compost. Eat lots of raw vegetables. Observe food combining rules (see Kulvinskas and Wigmore). Eat lots of prebiotics and probiotics, kombucha, kim chee, kraut, miso and natto. -- Lawrence F. London, Jr. lfljvenaura at gmail.com https://sites.google.com/site/avantgeared -------------- next part -------------- An HTML attachment was scrubbed... URL: From lfljvenaura at gmail.com Wed Feb 27 03:12:49 2019 From: lfljvenaura at gmail.com (Lawrence London) Date: Wed, 27 Feb 2019 03:12:49 -0500 Subject: [pchealth] =?utf-8?q?Exercise=2C_fasting_shown_to_help_cells_she?= =?utf-8?q?d_defective_proteins_=E2=80=93_Harvard_Gazette?= Message-ID: Exercise, fasting shown to help cells shed defective proteins ? Harvard Gazette https://news.harvard.edu/gazette/story/2019/02/exercise-fasting-shown-to-help-cells-shed-defective-proteins/ -------------- next part -------------- An HTML attachment was scrubbed... URL: From lfljvenaura at gmail.com Wed Feb 27 03:20:28 2019 From: lfljvenaura at gmail.com (Lawrence London) Date: Wed, 27 Feb 2019 03:20:28 -0500 Subject: [pchealth] =?utf-8?q?Exercise=2C_fasting_shown_to_help_cells_she?= =?utf-8?q?d_defective_proteins_=E2=80=93_Harvard_Gazette?= Message-ID: Exercise, fasting shown to help cells shed defective proteins ? Harvard Gazette https://news.harvard.edu/gazette/story/2019/02/exercise-fasting-shown-to-help-cells-shed-defective-proteins/ Health & Medicine Exercise, fasting help cells shed defective proteins Researchers have found that vigorous exercise, fasting, and hormones improve elimination of toxic, misfolded, unnecessary proteins in mouse and human cells. Discovery of previously unknown mechanism may someday aid in battle against Alzheimer?s By *Ekaterina Pesheva* HMS Communications DateFebruary 21, 2019 The body?s ability to adapt to changing conditions and shifting physiologic demands is essential to its survival. To ensure cellular performance and the health of the entire organism, each cell must be able to dispose of damaged or unnecessary proteins. Now, a study from the Blavatnik Institute at Harvard Medical School (HMS) shows that intense exercise, fasting, and an array of hormones can activate cells? built-in protein-disposal systems and enhance their ability to purge defective, toxic, or unneeded proteins. The findings, published Feb. 19 in PNAS , reveal a previously unknown mechanism that is triggered by fluctuations in hormone levels, which signal changes in physiologic conditions. ?Our findings show that the body has a built-in mechanism for cranking up the molecular machinery responsible for waste-protein removal that is so critical for the cells? ability to adapt to new conditions,? said Alfred Goldberg , senior author on the study and professor of cell biology at the Blavatnik Institute. *Cellular housecleaning in disease and health* Malfunctions in the cells? protein-disposal machinery can lead to the accumulation of misfolded proteins, which clog up the cell, interfere with its functions, and, over time, precipitate the development of diseases, including neurodegenerative conditions such as amyotrophic lateral sclerosis and Alzheimer?s. The best-studied biochemical system used by cells to remove junk proteins is the ubiquitin-proteasome pathway. It involves tagging defective or unneeded proteins with ubiquitin molecules ? a process known as the ?kiss of death? ? marking them for destruction by the cell?s protein-disposal unit, known as 26S proteasome. The Daily Gazette Sign up for daily emails to get the latest Harvard news. Past research by Goldberg?s lab has shown that this machinery can be activated by pharmacological agents that boost the levels of a molecule known as cAMP, the chemical trigger that initiates the cascade leading to protein degradation inside cells, which in turn switches on the enzyme protein kinase A. The lab?s previous research found that cAMP-stimulating drugs enhanced the destruction of defective or toxic proteins, particularly mutant proteins that can lead to neurodegenerative conditions. The new findings, however, reveal that shifts in physiological states and corresponding changes in hormones can regulate this quality-control process independent of drugs. Goldberg?s lab previously focused on reining in overactive protein breakdown ? excessive protein removal that can cause muscle wasting in cancer patients or give rise to several types of muscle atrophy. In fact, a proteasome inhibitor drug Goldberg and his team developed to tamp down protein-disposal activity has been widely used to treat multiple myeloma, a common blood cancer marked by abnormal protein accumulation and overworked proteasomes. The team?s latest work, by contrast, is focused on developing therapies that do just the opposite ? invigorate the cell?s protein-disposal machinery when it is too sluggish. These newest findings open the door, at least conceptually, to precisely such treatments. ?We believe our findings set the stage for the development of therapies that harness the cells? natural ability to dispose of proteins and thus enhance the removal of toxic proteins that cause disease,? said study?s lead investigator, Jordan VerPlank , a postdoctoral research fellow in cell biology at the Blavatnik Institute. Such treatments may not necessarily involve the design of new molecules, but instead stimulate the cell?s built-in capacity for quality control. ?This is truly a new way of looking at whether we can turn up the cellular vacuum cleaner,? Goldberg said. ?We thought this would require the development of new types of molecules, but we hadn?t truly appreciated that our cells continually activate this process. ?The beauty and the surprise of it is that such new treatments may involve churning a natural endogenous pathway and harnessing the body?s pre-existing capacity to perform quality control,? he said. Finding our genomic clockwork Harvard researchers discover a biomarker that can determine both chronological and biological age [image: ?Although previous work has shown how intermittent fasting can slow aging, we are only beginning to understand the underlying biology,? said William Mair, associate professor at Harvard Chan School. Mitochondrial networks in the muscle cells of C. elegans (pictured) have been key elements in the study.] In pursuit of healthy aging Harvard study shows how intermittent fasting and manipulating mitochondrial networks may increase lifespan It is already well known that exercise has many salutary effects, but the researchers said the new findings hint at the possibility that exercise and fasting could also help reduce the risk of developing conditions associated with the accumulation of misfolded proteins, such as Alzheimer?s and Parkinson?s. That possibility, however, remains to be explored, the team noted. In their experiments, the researchers analyzed the effects of exercise on cells obtained from the thigh muscles of four human volunteers before and after vigorous biking. Following exercise, the proteasomes of these cells showed dramatically more molecular marks of enhanced protein degradation, including greater levels of cAMP. The same changes were observed in the muscles of anesthetized rats whose hind legs were stimulated to contract repeatedly. Fasting ? even for brief periods ? produced a similar effect on the cells? protein-breakdown machinery. Fasting increased proteasome activity in the muscle and liver cells of mice deprived of food for 12 hours, the equivalent of an overnight fast. In another round of experiments, the researchers exposed the liver cells of mice to glucago, the hormone that stimulates production of glucose as fuel for cells and tissues during periods of food deprivation or whenever blood sugar levels drop. The researchers observed that glucagon exposure stimulated proteasome activity and enhanced the cells? capacity to destroy misfolded proteins. Exposure to the fight-or-flight hormone epinephrine produced a similar effect. Epinephrine, also known as adrenaline, is responsible for stimulating the liver and muscle to mobilize energy reserves to boost heart rate and muscle strength during periods of physiologic stress. Liver cells treated with epinephrine showed marked increases in cAMP, as well as enhanced 26S proteasome activity and protein degradation. Epinephrine exposure also boosted proteasome activity ? a marker of protein degradation ? in the hearts of living rats. Similarly, when researchers exposed mouse kidney cells to vasopressin ? the antidiuretic hormone that helps the body retain water and prevents dehydration ? they observed higher levels of protein degradation as well. Taken together, these findings demonstrate that the rate of protein degradation can rise and fall swiftly in a variety of tissues in response to shifting conditions, and that such changes are mediated by fluctuations in hormone levels. This response was surprisingly rapid and short-lived, the scientists noted. For example, exposure to the antidiuretic hormone triggered protein breakdown in kidney cells within five minutes and subsided to pre-exposure levels within an hour, the experiments showed. The findings show that the diverse set of hormones that stimulate cAMP appear to share a common mechanism that alters the composition of cells. These have long been known to modify gene expression, but this latest research reveals they also play a critical role in cellular housecleaning by disposing of proteins that are no longer needed. *A new twist on a classic concept* The new findings build on observations about the physiologic effects of hormones first made by HMS physician Walter Cannon nearly a century ago and elegantly captured in his book ?The Wisdom of the Body? (1932). Some of Cannon?s most notable work includes defining the mechanism of action of epinephrine and its role in the fight-or-flight response. Epinephrine is one of the hormones whose action on the protein-disposal machinery is now illuminated by Goldberg?s latest work. In a symbolic coincidence, Goldberg?s lab occupies the very space where Cannon made his observations on the same hormone a hundred years ago. ?We think ours is truly a neoclassical discovery that builds on findings and observations made right here, in this very building, nearly a century ago,? Goldberg said. Study co-investigators included Jinghui Zhao and Sudarsanareddy Lokireddy, who is no longer at Harvard. The research was made possible through tissue samples provided by colleagues in Houston, Copenhagen, and Sydney. *The work was supported by grants from the National Institutes of Health?s National Institute of General Medical Sciences under grants R01 GM051923-20 and F32 GM128322, the Cure Alzheimer?s Fund, the Muscular Dystrophy Association (MDA-419143), Genentech, and Project ALS.* -------------- next part -------------- An HTML attachment was scrubbed... URL: