[NAFEX] rooting difficult -to-root cuttings using Agrobacteriumrhizogenes
marillen at earthlink.net
Sat Apr 30 21:19:54 EDT 2005
Agrobacterium rhizogenes is a naturally-occurring soil pathogen.
It is important to realize that while the wild-type bacterium is effective in inducing roots, the roots that form from such an infection are not desirable from a production standpoint. The scientists that are using this species have mostly worked with this bacterium as a proof-of-concept sort of thing. A more useful method of harnessing this bacterium is to first put it's root-inducing plant-hormone gene(s) under an inducible promoter so that the roots are initiated, but then the hormone can be turned off by the human and the roots can continue growth as "normal" roots. The bacteria themselves are destroyed, but since the plant growth is altered by insertion of specific bacterial genes into the plant chromosomes, the plant growth can subsequently be regulated without any bacteria present. Without knowledge and facilities to properly modify and contain this bacterium, its probably better to leave this "bug" alone. It and it's cousin A. tumefaciens are serious pests in their wild state. Plus, even though the wild Agrobacteria have been producing genetically-modified plants for millenia, there are pretty strict regulations about humans knowingly planting transgenic plants in the open without prior license to do so.
However, I do encourage you to seek out a local plant molecular biologist at your nearest university and talk to them about these fascinating and useful critters.
From: Mark Lee <markl at nytec.com>
Sent: Apr 29, 2005 4:18 PM
To: 'North American Fruit Explorers' <nafex at lists.ibiblio.org>
Subject: RE: [NAFEX] rooting difficult -to-root cuttings using Agrobacteriumrhizogenes
I have no idea. Hey NAFEX, where can we get some?
-Mark Lee, Seattle
From: nafex-bounces at lists.ibiblio.org
[mailto:nafex-bounces at lists.ibiblio.org] On Behalf Of bill dality
Sent: Friday, April 29, 2005 10:29 AM
To: North American Fruit Explorers
Subject: [NAFEX] rooting difficult -to-root cuttings using
where can i get some of this bug?
Mark Lee <markl at nytec.com> wrote:
I found this to be an interesting technical paper on rooting cuttings of
apple and pear trees.
"In vitro fruit trees rooting by Agrobacterium rhizogenes wild type
Does anyone have experience with Agrobacterium rhizogenes? Researchers
have found it useful for rooting cuttings of kiwis and walnuts. Is this
a common bacteria?
-Mark Lee, Seattle
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