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nafex - RE: [NAFEX] rooting difficult -to-root cuttings using Agrobacteriumrhizogenes

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  • From: Anton Callaway <marillen@earthlink.net>
  • To: North American Fruit Explorers <nafex@lists.ibiblio.org>
  • Subject: RE: [NAFEX] rooting difficult -to-root cuttings using Agrobacteriumrhizogenes
  • Date: Sat, 30 Apr 2005 21:19:54 -0400 (GMT-04:00)

Agrobacterium rhizogenes is a naturally-occurring soil pathogen.

It is important to realize that while the wild-type bacterium is effective in
inducing roots, the roots that form from such an infection are not desirable
from a production standpoint. The scientists that are using this species
have mostly worked with this bacterium as a proof-of-concept sort of thing.
A more useful method of harnessing this bacterium is to first put it's
root-inducing plant-hormone gene(s) under an inducible promoter so that the
roots are initiated, but then the hormone can be turned off by the human and
the roots can continue growth as "normal" roots. The bacteria themselves are
destroyed, but since the plant growth is altered by insertion of specific
bacterial genes into the plant chromosomes, the plant growth can subsequently
be regulated without any bacteria present. Without knowledge and facilities
to properly modify and contain this bacterium, its probably better to leave
this "bug" alone. It and it's cousin A. tumefaciens are serious pests in
their wild state. Plus, even though the wild Agrobacteria have been
producing genetically-modified plants for millenia, there are pretty strict
regulations about humans knowingly planting transgenic plants in the open
without prior license to do so.

However, I do encourage you to seek out a local plant molecular biologist at
your nearest university and talk to them about these fascinating and useful
critters.

Anton

-----Original Message-----
From: Mark Lee <markl@nytec.com>
Sent: Apr 29, 2005 4:18 PM
To: 'North American Fruit Explorers' <nafex@lists.ibiblio.org>
Subject: RE: [NAFEX] rooting difficult -to-root cuttings using
Agrobacteriumrhizogenes

I have no idea. Hey NAFEX, where can we get some?

-Mark Lee, Seattle



-----Original Message-----
From: nafex-bounces@lists.ibiblio.org
[mailto:nafex-bounces@lists.ibiblio.org] On Behalf Of bill dality
Sent: Friday, April 29, 2005 10:29 AM
To: North American Fruit Explorers
Subject: [NAFEX] rooting difficult -to-root cuttings using
Agrobacteriumrhizogenes



Hey mark,

where can i get some of this bug?

Mark Lee <markl@nytec.com> wrote:

I found this to be an interesting technical paper on rooting cuttings of
apple and pear trees.
"In vitro fruit trees rooting by Agrobacterium rhizogenes wild type
infection"
http://www.ejbiotechnology.info/content/vol1/issue3/full/4/4.PDF

Does anyone have experience with Agrobacterium rhizogenes? Researchers
have found it useful for rooting cuttings of kiwis and walnuts. Is this
a common bacteria?

-Mark Lee, Seattle

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  • RE: [NAFEX] rooting difficult -to-root cuttings using Agrobacteriumrhizogenes, Anton Callaway, 04/30/2005

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