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Re: [permaculture] Crisper gene technology - are there
Chronological
Thread
<
Chronological
>
<
Thread
>
From
: Steve Hart <stevenlawrencehart@gmail.com>
To
: permaculture <permaculture@lists.ibiblio.org>
Subject
: Re: [permaculture] Crisper gene technology - are there
Date
: Tue, 5 Jul 2016 15:49:36 +1200
Hi Declan...yes I did my masters in bed after breaking my back..a great way
to focus
By the way..there is plenty of those mongrels operating within the
Permaculture world too.
Steve Hart
On 5 July 2016 at 09:53, scrooge McDuck <declan@planetmail.net> wrote:
>
Well the reading I have done & stuff watched since my stroke has turned me
>
off GMOs big time. One of the few advantages of the stroke was that I
>
arrived in the 21st century. I discovered Permaculture. At night I watched
>
documentaries and torrents I had downloaded during the day rather than
>
suffer trash tv. So I became aware of the damage mankind is doing, and the
>
inherent evil of large super profitable businesses who focus on today's
>
profits rather than tomorrow's planet.
>
>
--
>
Declan Moriarty
>
>
Sent from my Android tablet with mail.com Mail. Please excuse my brevity.
>
On 04/07/2016, 21:20 Steve Hart <stevenlawrencehart@gmail.com> wrote:
>
>
> Yes Declan..it is another diversion from nature. What did we learn in
>
> Permaculture ? ? One that rings in my ears " work with nature not against
>
> her" because as we know...Mother nature always fights back. I've just
>
> started re-reading "Hidden Nature" by Bartholomew a biography on
>
> Schauberger...a must for all in the Permaculture network...Steve Hart
>
>
>
> On 5 July 2016 at 00:52, Lawrence London <lfljvenaura@gmail.com> wrote:
>
>
>
> > On Mon, Jul 4, 2016 at 5:45 AM, scrooge McDuck <declan@planetmail.net>
>
> > wrote:
>
> >
>
> > > What often horrifies me is where they get these 'crisper genes' from -
>
> > > often from sources we would do more than raise an eyebrow at. I have
>
> few
>
> > > facts, but I'm not comfortable with what I do know.
>
> > >
>
> > > Regards
>
> > >
>
> > > Declan Moriarty.
>
> > >
>
> > > *Sent:* Sunday, July 03, 2016 at 10:13 PM
>
> > > *From:* "Lawrence London" <lfljvenaura@gmail.com>
>
> > > *To:* permaculture <permaculture@lists.ibiblio.org>
>
> > > *Subject:* [permaculture] Crisper gene technology - are there
>
> > > (John d'Hondt: Will this new powerful gene modification technology
>
> have
>
> > > implications for permaculture, changing the genetic makeup pf
>
> generations
>
> > > of organisms, including those in the wild?)
>
> > >
>
> > > Crisper gene technology - Google Search
>
> > >
>
> >
>
>
https://www.google.com/search?q=Crisper+gene+technology&ie=utf-8&oe=utf-8
>
> > >
>
> >
>
> > From Wikipedia:
>
> >
https://en.wikipedia.org/wiki/CRISPR
>
> > CRISPR
>
> > From Wikipedia, the free encyclopedia
>
> > <
https://en.wikipedia.org/wiki/File:Crispr.png
>
>
> > Diagram of the CRISPR prokaryotic antiviral defense mechanism.[1]
>
> > <
https://en.wikipedia.org/wiki/CRISPR#cite_note-pmid20056882-1
>
>
> >
>
> > *Clustered regularly interspaced short palindromic repeats* (*CRISPR*,
>
> > pronounced *crisper*[2]
>
> > <
https://en.wikipedia.org/wiki/CRISPR#cite_note-sawyer2013-2
>) are
>
> > segments
>
> > of prokaryotic <
https://en.wikipedia.org/wiki/Prokaryotic
> DNA
>
> > <
https://en.wikipedia.org/wiki/DNA
> containing short repetitions of
>
> base
>
> > sequences. Each repetition is followed by short segments of "spacer DNA
>
> > <
https://en.wikipedia.org/wiki/Spacer_DNA
>" from previous exposures to
>
> a
>
> > bacteriophage virus or plasmid <
https://en.wikipedia.org/wiki/Plasmid
>
> >.[3]
>
> > <
https://en.wikipedia.org/wiki/CRISPR#cite_note-pmid20125085-3
>
>
> >
>
> > The *CRISPR/Cas system* is a prokaryotic immune system
>
> > <
https://en.wikipedia.org/wiki/Immune_system
> that confers resistance
>
> to
>
> > foreign genetic elements such as those present within plasmids
>
> > <
https://en.wikipedia.org/wiki/Plasmids
> and phages
>
> > <
https://en.wikipedia.org/wiki/Phage
>,[4]
>
> > <
https://en.wikipedia.org/wiki/CRISPR#cite_note-4
>[5]
>
> > <
https://en.wikipedia.org/wiki/CRISPR#cite_note-pmid17379808-5
>[6]
>
> > <
https://en.wikipedia.org/wiki/CRISPR#cite_note-pmid19095942-6
> and
>
> > provides a form of acquired immunity
>
> > <
https://en.wikipedia.org/wiki/Acquired_immunity
>. CRISPR spacers
>
> > recognize
>
> > and cut these exogenous genetic elements in a manner analogous to RNA
>
> > interference <
https://en.wikipedia.org/wiki/RNA_interference
> in
>
> > eukaryotic
>
> > <
https://en.wikipedia.org/wiki/Eukaryote
> organisms.[3]
>
> > <
https://en.wikipedia.org/wiki/CRISPR#cite_note-pmid20125085-3
> CRISPRs
>
> > are
>
> > found in approximately 40% of sequenced bacterial
>
> > <
https://en.wikipedia.org/wiki/Bacterial
> genomes and 90% of sequenced
>
> > archaea <
https://en.wikipedia.org/wiki/Archaea
>.[7]
>
> > <
https://en.wikipedia.org/wiki/CRISPR#cite_note-pmid17521438-7
>[note 1]
>
> > <
https://en.wikipedia.org/wiki/CRISPR#cite_note-8
>
>
> >
>
> > By delivering the Cas9 <
https://en.wikipedia.org/wiki/Cas9
> nuclease
>
> and
>
> > appropriate guide RNAs into a cell, the cell's genome
>
> > <
https://en.wikipedia.org/wiki/Genome
> can be cut at a desired
>
> location,
>
> > allowing existing genes to be removed and/or new ones added.[8]
>
> > <
https://en.wikipedia.org/wiki/CRISPR#cite_note-nature99-9
>[9]
>
> > <
https://en.wikipedia.org/wiki/CRISPR#cite_note-vb99-10
>[10]
>
> > <
https://en.wikipedia.org/wiki/CRISPR#cite_note-pmid26121415-11
>
>
> CRISPRs
>
> > have been used in concert with specific endonuclease
>
> > <
https://en.wikipedia.org/wiki/Endonuclease
> enzymes
>
> > <
https://en.wikipedia.org/wiki/Enzymes
> for genome editing
>
> > <
https://en.wikipedia.org/wiki/Genome_editing
> and gene regulation in
>
> > species throughout the tree of life
>
> > <
https://en.wikipedia.org/wiki/Tree_of_life_%28biology%29
>.[11]
>
> > <
https://en.wikipedia.org/wiki/CRISPR#cite_note-12
>
>
> >
>
> > Cas9 was the first nuclease discovered, followed by Cpf1, which was
>
> > discovered in the CRISPR/Cpf1 <
>
>
https://en.wikipedia.org/wiki/CRISPR/Cpf1
>
>
> > system of Francisella novicida
>
> > <
https://en.wikipedia.org/wiki/Francisella_novicida
>.[12]
>
> > <
https://en.wikipedia.org/wiki/CRISPR#cite_note-pmid26422227-13
>[13]
>
> > <
https://en.wikipedia.org/wiki/CRISPR#cite_note-pmid27096362-14
> Other
>
> > such
>
> > systems are thought to exist.[14]
>
> > <
https://en.wikipedia.org/wiki/CRISPR#cite_note-:3-15
>
>
> >
>
> > CRISPR/C2c2 from the bacterium Leptotrichia shahii
>
> > <
https://en.wikipedia.org/wiki/Fusobacteria
> is RNA-guided CRISPR
>
> system
>
> > that targets RNA rather than DNA, and can either cleave single-stranded
>
> RNA
>
> > targets or knock them down <
>
>
https://en.wikipedia.org/wiki/Gene_knockdown
>.
>
> > [15] <
https://en.wikipedia.org/wiki/CRISPR#cite_note-pmid27256883-16
>
>
> >
>
> > The CRISPR interference <
>
>
https://en.wikipedia.org/wiki/CRISPR_interference
>
> > >
>
> > technique has many potential applications, including altering the
>
> germline
>
> > <
https://en.wikipedia.org/wiki/Germline
> of humans, animals, and food
>
> > crops. The use of CRISPR for genome editing[16]
>
> > <
https://en.wikipedia.org/wiki/CRISPR#cite_note-17
>[17]
>
> > <
https://en.wikipedia.org/wiki/CRISPR#cite_note-18
> was the AAAS
>
> > <
>
> >
>
>
https://en.wikipedia.org/wiki/American_Association_for_the_Advancement_of_Science
>
> > >'s
>
> > choice for breakthrough of the year in 2015.[18]
>
> > <
https://en.wikipedia.org/wiki/CRISPR#cite_note-19
> Bioethical
>
> > <
https://en.wikipedia.org/wiki/Bioethical
> concerns have been expressed
>
> > about the prospect of using this nascent biotechnology for editing the
>
> > human germline.[19] <
https://en.wikipedia.org/wiki/CRISPR#cite_note-20
>
>
> > <>
>
> > Bitter fight over CRISPR patent heats up : Nature News & Comment
>
> > <
>
> >
>
>
http://www.nature.com/news/bitter-fight-over-crispr-patent-heats-up-1.17961
>
> > >
>
> > www.nature.com/news/bitter-fight-over-*crispr*-patent-heats-up-1.17961
>
> > Nature Jan 12, 2016 -
>
> > Use of the *technology* in research has exploded, thanks to
>
> *CRISPR*–Cas9's
>
> > relative simplicity and versatility compared to other gene-editing ...
>
> > <>
>
> >
http://www.radiolab.org/story/antibodies-part-1-crispr/
>
> > Hidden inside some of the world’s smallest organisms is one of the most
>
> > powerful tools scientists have ever stumbled across. It's a defense
>
> system
>
> > that has existed in bacteria for millions of years and it may some day
>
> let
>
> > us change the course of human evolution.
>
> > <>
>
> >
>
> > I listened to an interview on public radio last week with an (the?)
>
> > inventor of the CRISPR technology currently in intense development.
>
> > 1) He insisted and will campaign for development and use of CRISPR to be
>
> > completely in the public view, interactive with the public and not
>
> behind
>
> > closed doors. He said that this is the way forward for maximum progress
>
> > with and best use of CRISPR.
>
> > 2) He described it this way. With conventional gene splcing the GMO
>
> > organism can eventually shed the modifications to its genetic makeup.
>
> With
>
> > CRISPR it can not.because it inserts a gene segment plus a tool (spacer)
>
> > that keeps it in place and protects it from evolutionary changes. In
>
> other
>
> > words it makes permanent changes to an organism's genome such that, as
>
> any
>
> > organism modified with CRISPR reproduces, those changes are retained in
>
> any
>
> > offspring and for generations forward. [this is my take on what he said
>
> > several days ago - I tried to find the interview and name of the
>
> researcher
>
> > but without luck],
>
> >
>
> > So, Declan, what "questionable" materials are sourced for use in CRISPR.
>
> > BTW they are talking about modifications to the human genome with this
>
> > promising technology. .
>
> > --
>
> > Lawrence F. London
>
> > lfljvenaura@gmail.com
>
> >
https://sites.google.com/site/avantgeared/
>
> >
https://plus.google.com/u/0/b/116100117925365792988/+Avantgeared/posts
>
> >
https://ello.co/permaculture
>
> >
https://lists.ibiblio.org/sympa/arc/permaculture
>
> > permaculture mailing list
>
> > permaculture@lists.ibiblio.org
>
> > subscribe/unsubscribe|user config|list info:
>
> >
http://lists.ibiblio.org/mailman/listinfo/permaculture
>
> >
>
>
>
>
>
>
>
> --
>
> Kia ora Ehoa
>
>
>
> [image: Picture]
>
> *Steve Hart*
>
> *Ecology Architect*
>
> *Designer, Teacher, Builder of Ecologically Sustainable Resilient
>
> Environments, through Urban Design, Architecture, Landscape Architecture
>
> and Systems Ecology.*
>
> Skype: tipenemanawa
>
> Phone: +64 220 75 62 11
>
>
>
> Permaculture Global Design:
>
> www.permacultureglobaldesign.org
>
>
>
> The Permaculture International College
>
> www.permaculturecollege.eu
>
>
https://permaculturecollege.academia.edu/SteveHart
>
>
>
> GAPPS The Global Alliance for Permaculture Partnerships and Solutions.
>
> www.permaculturepartnerships.com
>
> permaculture mailing list
>
> permaculture@lists.ibiblio.org
>
> subscribe/unsubscribe|user config|list info:
>
>
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>
>
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--
Kia ora Ehoa
[image: Picture]
*Steve Hart*
*Ecology Architect*
*Designer, Teacher, Builder of Ecologically Sustainable Resilient
Environments, through Urban Design, Architecture, Landscape Architecture
and Systems Ecology.*
Skype: tipenemanawa
Phone: +64 220 75 62 11
Permaculture Global Design:
www.permacultureglobaldesign.org
The Permaculture International College
www.permaculturecollege.eu
https://permaculturecollege.academia.edu/SteveHart
GAPPS The Global Alliance for Permaculture Partnerships and Solutions.
www.permaculturepartnerships.com
[permaculture] Crisper gene technology - are there
,
Lawrence London, 07/03/2016
Re: [permaculture] Crisper gene technology - are there
,
scrooge McDuck, 07/04/2016
Re: [permaculture] Crisper gene technology - are there
,
Lawrence London, 07/04/2016
Re: [permaculture] Crisper gene technology - are there
,
Steve Hart, 07/04/2016
Re: [permaculture] Crisper gene technology - are there
,
Lawrence London, 07/04/2016
Re: [permaculture] Crisper gene technology - are there
,
Lawrence London, 07/04/2016
<Possible follow-up(s)>
Re: [permaculture] Crisper gene technology - are there
,
scrooge McDuck, 07/04/2016
Re: [permaculture] Crisper gene technology - are there
,
Steve Hart, 07/04/2016
Re: [permaculture] Crisper gene technology - are there
,
Toby Hemenway, 07/05/2016
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