[pbs] twin scaling quandry
Nolo Contendre
miaam at ars-grin.gov
Tue Dec 9 05:40:21 EST 2003
Hippeastrum produces a red phenolic compound which will color tissue culture
media and sometimes appears on twin scales (or other injured tissue).
Alan Meerow
----- Original Message -----
From: <Bonsaigai37 at aol.com>
To: <pbs at lists.ibiblio.org>
Sent: Monday, December 08, 2003 8:34 PM
Subject: [pbs] twin scaling quandry
> Hello Group,
>
> I have a question about twin scaling Hippeastrum. After following a
combined
> procedure by RHS and IBS, using Cleary's 3336F on the scales, in
sterilized
> perlite, I'm noticing an even pink coloration on the scales. I think,
hmmm,
> damnable Stagonospora... yet I used Bonomyl (Benlate sub) for soaking the
bulb
> prior to storage, Bleach (1:10) prior to cuttage, and 3336F for the final
soak,
> alcohol rinsed surfaces and sterile razors. Do Hippeastrum show red or
pink
> as a form or oxidation to damaged cells or is my culture simply
contaminated?
> Any remedies?
>
> OH... I just joined the list. I'm Michael Loos, Cleveland,
> Ohio-ly-mackerel-it's-cold. I grow all sorts of geophytes including
succulent caudiciforms,
> fat - in and out of the ground. I've lots and ones and twos (3's-4's) of
many
> species, but heavy on the genera of Hippeastrum and Euphorbia. Presently,
I'm
> building my species list of Hippeastrum. (Anyone with species Hipps for
sale
> or trade, please feel free to contact me.)
>
> Thanks for the information,
> Grow Well,
> Michael Loos
> Honest Earth Consultation
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