[NAFEX] Scion storage in freezer?
volkenin at mail.lib.msu.edu
Tue Jan 27 15:09:25 EST 2004
I am a librarian so I did some searching in the agriculture literature and came up with listings for two article that might have some value for someone wanting to do more research on the topic.
Michigan State University
East Lansing, MI 48824
Email: volkenin at msu.edu
volkenin at egr.msu.edu
Record 1 of 2 in CAB Abstracts 2000/08-2002/07
TI: Cryopreservation of apple (Malus X domestica Borkh.) shoot tips following
encapsulation-dehydration or encapsulation-vitrification.
AU: Paul-H; Daigny-G; Sangwan-Norreel-BS
SO: Plant-Cell-Reports. 2000, 19: 8, 768-774; 35 ref.
AB: Axillary shoot tips of apple cv. Golden Delicious isolated from shoot
cultures were successfully cryopreserved using the encapsulation-dehydration
technique. After encapsulation in alginate gel, embedded shoot tips were
dehydrated by exposure to a sterile air flow before being frozen in liquid
nitrogen and subsequent slow thawing. A preculture on modified MS medium
containing 0.75 M sucrose followed by 6 h of dehydration (21% residual water)
led to the highest shoot regrowth of frozen, coated shoot tips (83.7%). Among
the sugars tested, sucrose and sorbitol presented the best cryoprotective
effect. Four other scion apple varieties and rootstocks were also successfully
cryopreserved. Axillary shoot tips of five apple (Malus X domestica [M.
pumila]) scion and root-stock cultivars were cryopreserved using the
encapsulation-vitrification technique. Using a one-step freezing method, we
successfully cryopreserved axillary shoot tips without the requirement of a
cold hardening pretreatment of the shoot cultures. Cryopreserved shoot tips
treated with aqueous cryoprotective mixture IV containing 180% (w/v) sucrose
and 120% (v/v) ethylene glycol showed the highest shoot regrowth rates, which
varied from 64 to 77%, depending on the cultivar.
Record 2 of 2 in CAB Abstracts 1979-1981
TI: Long-term storage of apple scions.
AU: Haniuda-T; Yoshida-Y; Tsuchiya-S; Sanada-T
SO: Long-term-preservation-of-favourable-germ-plasm-in-arboreal-crops. 1978, 89
-96; 13 ref.
PB: Fruit Tree Research Station, Ministry of Agriculture and Forestry.; Ibaraki
AB: One-year-old dormant shoots (scion material) of several apple cvs (including
Fuji, McIntosh, Akane and Virginia Crab) and wild Malus spp. were stored at
various temperatures, following different fungicidal and pre-freezing
treatments. With ordinary low temperature (0 deg C) storage, Akane scions
wrapped in vinyl film stored well for up to 2 years and 50% were then grafted
successfully on M. prunifolia rootstocks. Dipping for 15 min in a solution of
pentachlorophenol solution gave complete protection against fungi although the
high concentration tested [unspecified] injured the shoot tissue. A gradual,
rather than rapid dehydration, to about 35-40% of the original moisture
content, prior to freezing at -80 deg , reduced shoot injury and improved
viability. Pre-freezing at -10 deg to -40 deg before freezing at -80 deg gave
the best results. Many buds and cortex tissues survived after 30 days in liquid
N and 70-90% of these buds were grafted successfully.
From: Mark Lee [mailto:markl at nytec.com]
Sent: Tuesday, January 27, 2004 2:40 PM
To: North American Fruit Explorers
Subject: RE: [NAFEX] Scion storage in freezer?
If no one in NAFEX is aware of any research on long-term storage of
scionwood in a block of ice, this seems like a good project for one of us to
do. Any volunteers? It wouldn't require any expensive equipment, and the
results would directly benefit our members. Is the quality any better than
the standard dip into bleach solution? Could you cut the scionwood in one
season, and then use it a year later and have the grafts take?
More information about the nafex